lateral flow

Using a strip of porous membrane material as a platform, we can employ our liposome technology in the detection of target analytes in a lateral flow assay format. This format lends itself for inexpensive, rapid and extremely simple to use devices for point of care diagnostics, resource-limited settings and alike. Employing liposomes in this format allows for the visual detection of as little as 1 fmol of RNA.

For example:

• Streif, S., Neckermann, P., Spitzenberg, C., Weiß, K., Hoecherl, K., Kulikowski, K., Hahner, S., Noelting, C., Einhauser, S., Peterhoof, D., Asam, C., Wagner, R., Baeumner, A.J. “Liposome-based high-throughput and point-of-care assays toward the quick, simple, and sensitive detection of neutralizing antibodies against SARS-CoV-2 in patient sera.“ Analytical and Bioanalytical Chemistry (2023) 415, 1421 – 1435, Issue Cover, Paper in Forefront, DOI: 10.1007/s00216-023-04548-3
• Frohnmeyer E, Tuschel N, Sitz T, Hermann C, Dahl GT, Schulz F, Baeumner AJ, Fischer M “Aptamer Lateral Flow Assays for Rapid and Sensitive Detection of Cholera Toxin” Analyst (2019) 144:1840–1849, DOI: 10.1039/C8AN01616J

• Edwards, K.A., Baeumner, A.J. “Liposome-enhanced Lateral-flow Assays for the Sandwich-Hybridization Detection of RNA” Book chapter in “Biosensors and Biodetection: Methods and Protocols volume 2” Humana Press Books and Journals, Editors Avraham Rasooly, Keith E. Herold, pp. 185 - 215 (2009), DOI: 10.1007/978-1-60327-569-9_13

• Connelly, J.T., Nugen, S.R., Borejsza-Wysocki, W., Durst, R.A., Montagna, R.A., Baeumner, A.J. “Human Pathogenic Cryptosporidium species bioanalytical detection method with single oocyst detection capability”, Analytical and Bioanalytical Chemistry Issue cover vol. 391 (2), pp. 487 – 495 (2008), DOI: 10.1007/s00216-008-1967-2

• Baeumner, A.J., Leonard, B., McElwee, J., Montagna, R.A. “A Rapid Biosensor for Viable B. anthracis Spores” Analytical Bioanalytical Chemistry, vol. 380 (1), p. 15 – 23 (2004), DOI: 10.1007/s00216-004-2726-7

• Zaytseva, N.V., Montagna, R.A., Lee, E.M., Baeumner, A.J. “Multi-Analyte Single-Membrane Biosensor for Serotype Specific Detection of Dengue Virus” Analytical Bioanalytical Chemistry, vol. 380 (1), p. 46 – 53 (2004), DOI: 10.1007/s00216-004-2724-9

• Baeumner, A.J., Cohen, R.N., Miksic, V., Min, J.H. “RNA Biosensor for the Rapid Detection of Viable Escherichia coli in Drinking Water” Biosensors and Bioelectronics, vol. 8 (4) pp. 405 – 419 (2003), https://www.ncbi.nlm.nih.gov/pubmed/12604258