Research
Research interests
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Physiology and patho-physiology of electrolyte transport in kidney, respiratory tract, and intestine.
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Patho-physiology and molecular biology of polycystic kidney disease.
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Patho-physiology and molecular biology of cystic fibrosis and other inflammatory airway diseases.
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Development of novel ways for the treatment of asthma and cystic fibrosis lung disease. Exploring the possibility of targeting alternative ion channels.
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Role of ion channels for cell proliferation and cancer.
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Role of ion channels in inflammatory diseases.
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Contribution of TMEM16 proteins to regulated cell death, e.g. ferroptosis, apoptosis, and pyroptosis.
Methods used
Electrophysiology
- Open and short circuit measurements in Ussing chambers
- Patch clamp techniques in all applications
Fluorescence based techniques
- Immunostaining of cellular components, soluble and membrane proteins in Epithelial and non-epithelial cells
- Fluorescence ratio measurements of intracellular pH, Ca2+ and Cl-
- Confocal microscopy and video imaging
- Iodide quenching of YFP fluorescence in plate reader and single cell microscopy
Cell biological techniques
- Primary and permanent cell culture of epithelial and non-epithelial cells
- S2 techniques including viral transfection, infectious agents
- Cell tracking by HoloMonitor M4 Microscopy
- Casy flow cytometry
- Accury flow cytometry 4 wavelengths
- Reconstitution of membrane proteins in liposomes
Protein biochemistry & Molecular biology
- Western blotting
- Immunoprecipitation, coimmunoprecipitation
- DNA-cloning techniques
- site directed mutagenesis
- PCR - applications
- DNA sequencing
- siRNA techniques
- CRISPR-Cas technology
Knockout technology
- In vivo and ex vivo animals studies
- Conventional and Cre/loxP knockout mice
- Inducible Cre/loxP knockout mice
AG Kunzelmann - 04.05.2021 09:31 
